Analysis Note
ControlPBMCs
Application
Research Sub CategoryImmunoglobulins & Immunology
Milli-Mark Anti-CD11B-PE Antibody, clone 2LPM19c detects level of CD11B & has been published & validated for use in FC.
Research CategoryInflammation & Immunology
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
CD11b is the 165 kDa α-chain of the Mac-1 complex. The molecule, also called CR3, functions as a cell surface receptor for the C3bi complement fragment. There is evidence that CD11b can bind fibrinogen in addition to C3bi (Wright,1988). Two binding sites have now been reported, one recognizing lipopolysaccharide, and the other protein ligands containing the arg-gly-asp sequence (Wright, 1989). Binding of the coagulation factor X has also been suggested as an additional receptor role of CD11b (Altieri,1988). This protein belings to a family of related heterodimeric proteins designated CD11 in the classification system for human leucocyte differentiation antigens, which shares a common β-chain with a MW of 95 kDa (CD18). Besides the C3bi receptor, this protein family contains two other members, leucocyte function associated-1 (LFA-1, C11a) antigen and p150,95 protein (CD11c) (Sanchez-Madrid,1983).
Immunogen
Purified iC3b receptor.
Legal Information
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Protein A purified
Purified Mouse monoclonal IgG1 conjugated to PE in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.
Quality
Evaluated by flow cytometry using human PBMCs
Specificity
Antibody recognizes Human CD11b antigen.
Storage and Stability
Maintained refrigerated at 2-8°C from date of receipt. Protected from light in undiluted aliquots for 6 months from date of receipt.
Target description
165 kDa (Wright, 1988)
This product has met the following criteria to qualify for the following awards: